Eindhoven University of Technology
Background:
The Nanoscopy for Nanomedicine group aims to use advanced optical imaging techniques (Nanoscopy) to understand at the molecular level the behavior of synthetic nanomaterials in vitro and in the biological environment (Nanomedicine). To achieve this goal they use a variety of methods such as super resolution localization microscopy (STORM, DNA‑PAINT, STED), single molecule imaging, single particle tracking, spectral imaging and correlative imaging we tailor and optimize such techniques to the study of engineered materials. They are currently employing these imaging methods to address key issues such as nanoparticle functional characterization, protein corona formation, materials stability in biological fluids, receptor targeting and intracellular trafficking.
In this framework, the understanding of the interactions of nanomaterials with biological matter is used to guide the rational design of materials for a variety of applications such as drug delivery, targeted therapies and biosensing.
Role in DESTINATION:
Throughout the project, the Nanoscopy for Nanomedicine group will utilise single molecule and super-resolution microscopy to assess aptamer cell internalisation. Additionally, they will employ novel methods based on multicolor single molecule imaging to evaluate RNanos intracellular localisation.
Key personnel:
Associate Prof. Lorenzo Albertazzi (PI), l.albertazzi@tue.nl